Title: The taming of small heat-shock proteins: crystallization of the alpha-crystallin domain from human Hsp27
Authors: Baranova, Ekaterina
Beelen, Steven
Gusev, N B
Strelkov, Sergei # ×
Issue Date: Dec-2009
Series Title: Acta Crystallographica F, Structural Biology and Crystallization Communications Online vol:65 issue:Pt 12 pages:1277-1281
Abstract: Small heat-shock proteins (sHsps) are ubiquitous molecular chaperones. sHsps function as homooligomers or heterooligomers that are prone to subunit exchange and structural plasticity. Here, a procedure for obtaining diffraction-quality crystals of the alpha-crystallin domain of human Hsp27 is presented. Initially, limited proteolysis was used to delineate the corresponding stable fragment (residues 90-171). This fragment could be crystallized, but examination of the crystals using X-rays indicated partial disorder. The surface-entropy reduction approach was applied to ameliorate the crystal quality. Consequently, a double mutant E125A/E126A of the 90-171 fragment yielded well ordered crystals that diffracted to 2.0 A resolution.
ISSN: 2053-230X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biocrystallography
× corresponding author
# (joint) last author

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