Title: Breakdown of thermodynamic equilibrium for DNA hybridization in microarrays
Authors: Hooyberghs, J ×
Baiesi, Marco
Ferrantini, Alessandro
Carlon, Enrico #
Issue Date: Jan-2010
Publisher: Published by the American Physical Society through the American Institute of Physics
Series Title: Physical Review E, Statistical, Nonlinear and Soft Matter Physics vol:81 issue:1 pages:1-4
Article number: 012901
Abstract: Test experiments of hybridization in DNA microarrays show systematic deviations from the equilibrium isotherms. We argue that these deviations are due to the presence of a partially hybridized long-lived state, which we include in a kinetic model. Experiments confirm the model predictions for the intensity vs free-energy behavior. The existence of slow relaxation phenomena has important consequences for the specificity of microarrays as devices for the detection of a target sequence from a complex mixture of nucleic acids.
ISSN: 1539-3755
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Theoretical Physics Section
× corresponding author
# (joint) last author

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