Title: Albumin as an adjunct to tracheal occlusion in fetal rats with congenital diaphragmatic hernia: a placebo-controlled study
Authors: Klaritsch, Philipp ×
Mayer, Steffi
Sbragia, Lourenço
Toelen, Jaan
Roubliova, Xenia
Lewi, Paul
Deprest, Jan #
Issue Date: Feb-2010
Publisher: Mosby, Inc.
Series Title: American Journal of Obstetrics and Gynecology vol:202 issue:2 pages:198.e1-9
Abstract: OBJECTIVE: We sought to investigate effects of intratracheal albumin injection prior to tracheal occlusion (TO) on lung proliferation in fetal rats with nitrofen-induced congenital diaphragmatic hernia. STUDY DESIGN: On embryonic day 19, nitrofen-exposed fetuses underwent TO, TO and 50 microL of either intratracheal albumin 20% or saline, or remained untouched. Main outcome at embryonic day 21.5 was expression of the proliferation marker Ki-67. Secondary outcomes were lung-to-bodyweight ratio (LBWR), tropoelastin expression, density and spatial distribution of elastin, pulmonary/alveolar morphometry, and fetal survival. RESULTS: TO increased Ki-67 messenger RNA and LBWR. Albumin further increased LBWR and density of Ki-67-positive cells but also fetal mortality. TO with or without adjuncts induced elastin deposits at the tips of arising secondary crests, increased air space size, and decreased septal thickness. CONCLUSION: TO had effects on lung proliferation and advanced the morphologic appearance. Addition of albumin increased density of proliferating cells and LBWR, yet at the expense of additional fetal loss.
ISSN: 0002-9378
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Foetal Medicine Section (-)
Molecular Virology and Gene Therapy
Section Woman - Miscellaneous (-)
× corresponding author
# (joint) last author

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