Title: The effect of maternal betamethasone and fetal tracheal occlusion on pulmonary vascular morphometry in fetal rabbits with surgically induced diaphragmatic hernia: a placebo controlled morphologic study
Authors: Roubliova, Xenia I ×
Lewi, Paul J
Verbeken, Eric
Vaast, Pascal
Jani, Jacques C
Lu, Huiqi
Tibboel, Dick
Deprest, Jan #
Issue Date: Jul-2009
Publisher: John Wiley & Sons
Series Title: Prenatal Diagnosis vol:29 issue:7 pages:674-681
Abstract: OBJECTIVES: We studied the vascular effects of betamethasone (BM) and/or tracheal occlusion (TO) in fetal rabbits with surgically induced congenital diaphragmatic hernia (CDH). METHODS: At day 23 (pseudoglandular phase; term = 31 d), 54 ovarian-end fetuses from 27 does underwent induction of CDH. Thirteen did receive either 0.05 mg/kg BM, on days 28 and 29 with a 24-h interval, or 14 saline [controls (CTR)]. At day 28, one ovarian-end fetus underwent TO and harvesting was at term. In total, we compared (ANOVA) lung-to-body weight ratio (LBWR) and vascular morphometric indices in survivors from the following groups (n - number alive at delivery): CDH (9); CDH + TO (10); unoperated controls (14); CDH + BM (10); CDH + TO (9); controls CTR + BM (13). RESULTS: Maternal BM had no effect on LBWR. LBWR was comparable to normal in CDH fetuses undergoing TO. Both TO and BM have an effect on medial thickening due to CDH which is larger when both interventions are combined. CONCLUSIONS: Both TO and BM lessen peripheric muscularization present in CDH lungs and their effect is cumulative.
ISSN: 0197-3851
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Foetal Medicine Section (-)
Translational Cell & Tissue Research
× corresponding author
# (joint) last author

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