Title: Production of tailor made short chain amylose-lipid complexes using varying reaction conditions
Authors: Putseys, Joke ×
Derde, Liesbeth
Lamberts, Lieve
Goesaert, Hans
Delcour, Jan #
Issue Date: Nov-2009
Publisher: Elsevier sci ltd
Series Title: Carbohydrate polymers vol:78 issue:4 pages:854-861
Abstract: When amylose was synthesized using potato phosphorylase in the presence of amylose complexing lipids, monodisperse populations of amylose-lipid complexes were formed. Enzyme dosage and glucose-1-phosphate (glc-1-P)/primer ratio influenced the reaction rate of the enzymic synthesis, presumably by changing the balance between amylose synthesis and amylose-lipid complexation and precipitation, and impacted the molecular weight of the complexes. Lipid characteristics affected the dissociation properties and amylose chain lengths of the amylose-lipid complexes presumably by determining the minimal amylose chain length necessary for complexation and precipitation. Tailor made short chain amylose-lipid complexes can hence be produced by choosing the appropriate reaction conditions. We propose a synthesis mechanism in which the primer is elongated until an amylose chain is obtained which is of sufficient length to complex a first lipid. Further chain extension then occurs, together with subsequent complexation until the complex becomes insoluble and precipitates. (C) 2009 Elsevier Ltd. All rights reserved.
ISSN: 0144-8617
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Intellectual Property
× corresponding author
# (joint) last author

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