Title: Intra- and inter-observer analysis in the morphological assessment of early-stage embryos
Authors: Paternot, Goedele ×
Devroe, Johanna
Debrock, Sophie
D'Hooghe, Thomas
Spiessens, Carl #
Issue Date: 29-Sep-2009
Publisher: BioMed Central
Series Title: Reproductive Biology and Endocrinology vol:7 issue:1 pages:105-105
Abstract: BACKGROUND: The aim of this study was to determine the intra- and inter-observer variability in the evaluation of embryo quality. Multilevel images of embryos on day 1, day 2 and day 3, were analysed using different morphological parameters. METHODS: Multilevel images of embryos on day 1, day 2 and day 3, were analysed using a standard scoring system. The kappa coefficient was calculated to measure intra- and inter-observer variability before and after training sessions. RESULTS: Good to excellent intra-observer agreement was present for most parameters exceptions being scoring the position of pronuclei and the presence of a cytoplasmic halo on day 1, multinucleation on day 2 and the size of fragments on day 3. Inter-observer agreement was only good to excellent for the number of blastomeres on day 2 and day 3 and the orientation of the cleavage axes on day 2. Training sessions had a positive impact on inter-observer agreement. CONCLUSION: In conclusion, assessment of morphological characteristics of early stage embryos using multilevel images was marked by a high intra-observer and a moderate inter-observer agreement. Training sessions were useful to increase inter-observer agreement.
ISSN: 1477-7827
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Reproductive Medicine Section (-)
Section Woman - Miscellaneous (-)
× corresponding author
# (joint) last author

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