S100A1 decreases calcium spark frequency and alters their spatial characteristics in permeabilized adult ventricular cardiomyocytes
Voelkers, Mirko * × Loughrey, Christopher M * Macquaide, Niall Remppis, Andrew DeGeorge, Brent R Wegner, Frederic V Friedrich, Oliver Fink, Rainer H. A Koch, Walter J Smith, Godfrey L Most, Patrick #
Cell calcium vol:41 issue:2 pages:135-143
S100Al, a Ca2+-sensor protein of the EF-hand type, exerts positive inotropic effects in the heart via enhanced cardiac ryanodine receptor (RyR2) activity. Here we report that S100Al protein (0.1 mu M) interacts with the RyR2 in resting permeabilized cardiomyocytes at free Ca2+- levels comparable to diastolic Ca2+-concentrations (similar to 150 nM). Alterations of RyR2 function due to S100Al binding was assessed via analysis of Ca2+-spark characteristics. Ca2+-spark frequency, amplitude and duration were all reduced upon perfusion with 0. 1 mu M S100Al protein by 38%, 14% and 18%, respectively. Most likely, these effects were conveyed through the S100Al C-terminus (S100Al-ct; amino acids 75-94) as the corresponding S100Al-ct peptide (0.1 mu M) inhibited S100Al protein binding to the RyR2 andsimilarly attenuated frequency, amplitude and duration of Ca2+-sparks by 52%, 8% and 26%, respectively. Accordingly, the sarcoplasmic reticulum (SR) Ca2+-content was slightly increased but the stoichiometry of other accessory RyR2 modulators (sorcin/FKBP12.6) remained unaltered by S100Al. Hence, we propose S100Al as a novel inhibitory modulator of RyR2 function at diastolic Ca2+-concentrations in rabbit ventricular cardiomyocytes. (c) 2006 Published by Elsevier Ltd.