Title: Cardiotin localization in mitochondria of cardiomyocytes in vivo and in vitro and its down-regulation during dedifferentiation
Authors: Driesen, Ronald ×
Verheyen, Fons K
Schaart, Gert
De Maziere, Ann
Viebahn, Christoph
Prinzen, Frits W
Lenders, Marie-Helene
Debie, Wiel
Totzeck, Andreas
Borgers, Marcel
Ramaekers, Frans C. S #
Issue Date: Jan-2009
Publisher: Elsevier science inc
Series Title: Cardiovascular pathology vol:18 issue:1 pages:19-27
Abstract: Background: Cardiotin expression is observed in adult cardiac tissue. In the present study, we provide evidence for the specific localization of cardiotin in cardiac mitochondria and for its down-regulation during adaptive remodeling (dedifferentiation) of cardiomyocytes. Methods: Immunocytochemistry was used to study cardiotin localization in adult rabbit papillary muscle, in late-stage embryonic rabbit left ventricular tissue, and in left ventricle samples of rabbits suffering from pressure and volume overload. Western blot analysis of cardiotin was performed in purified pig heart mitochondrial fractions. Cardiotin expression was monitored in vitro in isolated adult rat and rabbit left ventricular cardiomyocytes. Results: Western blot analysis revealed the presence of cardiotin in the mitochondrial fractions of pig heart. Immunoelectron microscopy confirmed the presence of cardiotin in cardiac mitochondria of normal adult rabbits both in vivo and in vitro. Quantification of the localization of immunogold particles Suggests an association of cardiotin with the mitochondrial inner membrane. Cardiotin expression is initiated in late-stage ernbryonic rabbit heart, whereas in adult ventricular tissue cardiotin clearly stained longitudinal arrays of mitochondria. Pressure- and volume-overloaded myocardium showed a reduction in cardiotin expression in dispersed local myocardial areas. Cell cultures of adult cardiomyocytes showed a gradual loss in cardiotin expression in parallel with a sarcomeric remodeling. Conclusions: Our results demonstrate the specific localization of cardintin in adult cardiomyocyte mitochondria and propose its use as an early marker for cardiomyocyte adaptive remodeling and dedifferentiation. (C) 2009 Elsevier Inc. All rights reserved.
ISSN: 1054-8807
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Experimental Cardiology
× corresponding author
# (joint) last author

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