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Title: Species identification of corynebacteria by cellular fatty acid analysis
Authors: Van den Velde, Sandra ×
Lagrou, Katrien
Desmet, Koenraad
Wauters, Georges
Verhaegen, Jan #
Issue Date: Feb-2006
Series Title: Diagnostic microbiology and infectious disease vol:54 issue:2 pages:99-104
Abstract: We evaluated the usefulness of cellular fatty acid analysis for the identification of corynebacteria. Therefore, 219 well-characterized strains belonging to 21 Corynebacterium species were analyzed with the Sherlock System of MIDI (Newark, DE). Most Corynebacterium species have a qualitative different fatty acid profile. Corynebacterium coyleae (subgroup 1), Corynebacterium riegelii, Corynebacterium simulans, and Corynebacterium imitans differ only quantitatively. Corynebacterium afermentans afermentans and C. coyleae (subgroup 2) have both a similar qualitative and quantitative profile. The commercially available database (CLIN 40, MIDI) identified only one third of the 219 strains correctly at the species level. We created a new database with these 219 strains. This new database was tested with 34 clinical isolates and could identify 29 strains correctly. Strains that remained unidentified were 2 Corynebacterium aurimucosum (not included in our database), 1 C. afermentans afermentans, and 2 Corynebacterium pseudodiphtheriticum. Cellular fatty acid analysis with a self-created database can be used for the identification and differentiation of corynebacteria.
URI: 
ISSN: 0732-8893
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Periodontology
Laboratory of Clinical Bacteriology and Mycology
× corresponding author
# (joint) last author

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