Title: Epigenetic control of aquaporin 1 expression by the amyloid precursor protein
Authors: Huysseune, Sandra
Kienlen-Campard, Pascal
Hébert, Sébastien
Tasiaux, Bernadette
Leroy, Karelle
Devuyst, Olivier
De Strooper, Bart
Octave, Jean-Noel # ×
Issue Date: Dec-2009
Publisher: The Federation of American Societies for Experimental Biology
Series Title: FASEB Journal vol:23 issue:12 pages:4158-4167
Abstract: Cellular processing of the amyloid precursor protein (APP) has been extensively studied, but its precise function remains elusive. The intracellular domain of APP has been proposed to regulate expression of several genes by mechanisms that are largely unknown. We report that APP regulates expression of the aquaporin 1 (AQP1) gene in mouse embryonic fibroblasts and in transgenic mice. AQP1 mRNA and protein were down-regulated in fibroblasts lacking APP or presenilin 2 in which AQP1 expression was restored by stable expression of full-length APP or presenilin 2 but not by APP deleted from its carboxy-terminal domain. The transcriptional activity of the AQP1 gene promoter and the stability of AQP1 mRNA were identical in fibroblasts expressing or not expressing APP. Control of AQP1 expression by APP was sensitive to trichostatin A, an histone deacetylase inhibitor, and histone deacetylase activity coimmunoprecipitated with APP. Altogether, these data show that a presenilin-2-dependent gamma-secretase activity releases the intracellular domain of APP involved in the epigenetic control of AQP1 expression. Since AQP1 is found in astrocytes surrounding senile plaques, this epigenetic control of AQP1 expression could have important implications in Alzheimer disease.-Huysseune, S., Kienlen-Campard, P., Hebert, S., Tasiaux, B., Leroy, K., Devuyst, O., Brion, J.-P., De Strooper, B., Octave, J.-N. Epigenetic control of aquaporin 1 expression by the amyloid precursor protein. FASEB J. 23, 4158-4167 (2009).
ISSN: 0892-6638
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Molecular Genetics Section (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Huysseune et al_FasebJ.pdf Published 352KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science