Title: An integrated molecular and histological study on the impact of sublethal effects of Fenoxycarb on the European honeybee, Apis mellifera
Authors: Heylen, Kevin
Billen, Johan
Huybrechts, Roger #
Issue Date: Jul-2008
Conference: The International Congress of Entomology location:Durban, South Africa date:July 2008
Abstract: The aim of our research was to investigate if a synthetic Juvenile hormone (JH)-analog (the insecticide Fenoxycarb) potentially affects physiological processes in the honeybee. We evaluated effects on both the molecular level (gene and protein expression in the brain) and the organ level (hypopharyngeal gland development). Both mRNA and protein expression profiles were analysed in age-matched treated and control bees to identify relevant
components using microarray and 2D-DIGE (2-dimensional fluorescence difference gel electrophoresis). Analysis of brains resulted in a list of 21 genes that were consistently
differentially expressed in microarray experiments for 2 different colonies, while 7 differential protein spots were shared in 2D-DIGE experiments on 3 different colonies. So far, four protein spots were successfully identified using MS, of which two also appeared in the microarray list. When we focused on a specific gene involved in the transition from nurse to forager, bees treated with the JH-analog showed different expression to control bees. Moreover, histological studies on the important and JH-sensitive hypopharyngeal glands were also performed to look for morphological differences between treated and control bees. Age related degeneration of the hypopharyngeal gland progressed faster in bees, treated with Fenoxycarb, in comparison to control bees. Our data show that effects of JH-analogs on bees can be detected at the molecular and developmental level which provides a potential tool for
side-effect evaluation.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Research Group Neuroplasticity and Neuroproteomics (-)
Ecology, Evolution and Biodiversity Conservation Section
# (joint) last author

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