Title: Selection of an immunohistochemical panel for cardiovascular research in sheep
Authors: De Visscher, Geofrey ×
Plusquin, Ruth
Mesure, Lindsay
Flameng, Willem #
Issue Date: Jul-2010
Publisher: Lippincott Williams & Wilkins
Series Title: Applied Immunohistochemistry & Molecular Morphology vol:18 issue:4 pages:382-391
Abstract: Large animal research, often required as a final phase before commencing clinical trials for devices, has generally been hampered by the lack of appropriate tools to compare it with either initial small animal tests or to later evaluation in humans. Setting out to tissue engineer heart valves, we were particularly struck by the limited availability of immunohistochemical markers for sheep tissue, despite sheep being the FDA-approved animal for heart valve testing. This paper, therefore, aims to compile the available knowledge and extend the marker list with antibodies cross-reacting with sheep tissue. Thirty-seven antibodies attributed to 1 of these classes were found to be useful: (1) endothelium, (2) mesenchymal cells, myofibroblasts, and smooth muscle cells, (3) immune response, (4) primitive cells, (5) extracellular matrix, and (6) miscellaneous. Twelve had already been used in sheep tissue, but to our knowledge, the remaining 25 have not been described for use in sheep. From this result, we can conclude that the immunohistochemical panel for sheep has been extensively expanded with respect to cardiovascular research.
ISSN: 1541-2016
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Experimental Cardiac Surgery
× corresponding author
# (joint) last author

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