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Title: Analysis of Mitochondrial pH and Ion Concentrations
Authors: Vandeven, Martin ×
Balut, Corina
Baron, Szilvia
Smets, Ilse
Steels, Paul
Ameloot, Marcel #
Issue Date: Jan-2010
Series Title: Methods in molecular biology (Clifton, N.J.) vol:591 pages:275-309
Abstract: Detailed practical information is provided with emphasis on mapping cytosolic and mitochondrial pH, mitochondrial Na(+), and briefly also aspects related to mitochondrial Ca(2+) measurements in living cells, as grown on (un)coated glass coverslips. This chapter lists (laser scanning confocal) microscope instrumentation and setup requirements for proper imaging conditions, cell holders, and an easy-to-use incubator stage. For the daily routine of preparing buffer and calibration solutions, extensive annotated protocols are provided. In addition, detailed measurement and image analysis protocols are given to routinely obtain optimum results with confidence, while avoiding a number of typical pitfalls.
ISSN: 1064-3745
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Cellular Transport Systems
× corresponding author
# (joint) last author

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