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Title: The differential character of protein expression in the developing mouse brain and beyond: a proteomic approach
Authors: Van de Plas, Babs
Van Hove, Inge
Clerens, Stefan
Van den Bergh, Gert
Arckens, Lut #
Issue Date: Oct-2006
Host Document: Soc. Neurosci. Abstr., 2006
Conference: Annual Meeting of the Society for Neuroscience edition:36 location:Atlanta, GA, U.S.A. date:October 14-18, 2006
Article number: 786.17
Abstract: In mammalian brain sensory systems have the remarkable capacity to adapt to input changes by modifying neuronal connectivity within a ‘critical period’ early in life. Goal-directed strengthening, remodelling and elimination of synapses build the adult specific neuronal circuitry. In adulthood, this connectional plasticity appears greatly reduced.
We investigated the age-dependent (dis)similarities in molecular mechanisms underlying these age-dependent differences of brain plasticity in young and adult mice by means of fluorescent two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry (MS). With 2D-DIGE, protein samples from whole forebrain extracts of 10-day-old pups (P10) and adult mice (3 months old, A3) were compared. Approximately 140 differentially expressed proteins were identified by MS. Apart from metabolic enzymes, the list contained proteins involved in the outgrowth of axons, growth cone collapse, and neuronal differentiation. Proteins selected for further validation via Western blotting were dynamin-1 and NSE, both upregulated in adult brain, and fascin, more abundantly expressed in pup. We compared the expression levels of these 3 proteins between P1, P5, P10, P30, A3 and A7 mice. These results confirmed our 2D-findings. The distribution pattern of fascin was studied with immunocytochemistry on coronal sections of the entire mouse brain (P10 & A3). Laminar staining patterns were observed which differed between adjacent areas and which allow us to distinguish several cortical regions. At P10, layers II, III, and V showed a dominant neuropil staining. Immunopositive neurons were observed here, and in layer VI to a lesser extent. In adult brain, neuropil staining was also observed in layers II, III, and V, moreover in layer IV, around bregma -0.1, at the border with the infragranular layers. The fascin antibody stained neurons in layers II and III with an immunopositive perikaryon and proximal part of the apical dendrite, but no immunostaining was seen in the nucleus.
Our results clearly indicate typical age-dependent differences in protein expression patterns in mouse brain during development.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Research Group Neuroplasticity and Neuroproteomics (-)
# (joint) last author

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