|ITEM METADATA RECORD
|Title: ||Effect of binocular but not monocular deprivation on the developmental expression pattern and activity of cyclin-dependent kinase 5 in cat visual cortex|
|Authors: ||Van den Bergh, Gert|
Arckens, Lut #
|Issue Date: ||Oct-2006 |
|Host Document: ||Soc. Neurosci. Abstr., 2006|
|Conference: ||Annual Meeting of the Society for Neuroscience edition:36 location:Atlanta, GA, U.S.A. date:October 14-18, 2006|
|Article number: ||619.5|
|Abstract: ||The structural and functional development of cat visual cortex is highly dependent of visual experience early in life. Monocular deprivation during this critical period results in a drastic rewiring of the primary visual cortex, while early binocular deprivation impairs binocularly driven aspects of visual perception. Proteomics studies in our laboratory demonstrated that several proteins showed differential expression in cat primary visual cortex during postnatal development. Several of these proteins, e.g. collapsin response mediator protein 2 (CRMP2), septin 5 and dynamin 1, are substrates of cyclin-dependent kinase 5 (cdk5), a neuronal protein involved in regulating a diverse range of cellular functions, including axon guidance, cytoskeletal stability, synaptic function, cell adhesion and apoptosis. Here we investigated by Western blotting the time-dependent expression of cdk5 and its activator p35 at eye-opening (P10), the peak and end of the critical period (P30 and P120) and in adult cats, both in normal animals and animals binocularly deprived from patterned vision from P10 (BD).
While the p35 activator protein level did not change during postnatal development, cdk5 showed an expression peak at P30 and P120. Similarly, two cdk5 substrates - septin 5 and the transcription factor MEF2A (myocyte enhancer factor 2A) - also showed higher expression at P30 compared to adult visual cortex, but only septin 5 was also more abundantly expressed at P120. In BD kittens, the expression of cdk5 was not changed compared to animals with normal visual development, but the p35 protein level showed a fourfold increase in BD cats at P30, indicating an increase in cdk5 activity. The expression levels of both septin 5 and MEF2A in BD animals also showed an increase at P30, while at other time-points their levels were unaltered compared to normal cats, thus reflecting the changed cdk5 activity in BD cats. In kittens monocularly deprived from P10 to P30, no expression level changes for any of the investigated proteins were observed.
Our results indicate that cdk5 could play a role in mediating the functional changes in the visual cortex, observed after pattern vision deprivation, by modulation of its activity.
|Publication status: ||published|
|KU Leuven publication type: ||IMa|
|Appears in Collections:||Animal Physiology and Neurobiology Section - miscellaneous|
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