Annual Meeting of the Society for Neuroscience edition:35 location:Washington DC, U.S.A. date:November 12-16, 2005
The structural and functional development of cat visual cortex is highly dependent of visual experience early in life. Monocular deprivation during this critical period results in a drastic rewiring of the primary visual cortex. Early binocular deprivation can delay the onset of the critical period and impairs binocularly driven aspects of visual perception.
Here, we investigated the effect of early binocular visual deprivation on the protein expression levels in cat primary visual cortex, by comparing protein expression levels from visual area 17 of normal 10-day-old and 30-day-old kittens and binocularly deprived 30-day-old kittens. Fluorescent two-dimensional difference gel electrophoresis (2-D DIGE) enabled the displaying and quantitative analysis of protein expression, whereas identification of the differentially expressed proteins was performed by Q-TOF mass spectrometry. Seven proteins differentially expressed between binocularly deprived and control 30-day-old kittens are transferrin, 14-3-3 proteins alpha/beta and gamma, septin 5, HMGC synthase and two collapsin response mediator proteins, CRMP2 and CRMP4. These same protein spots displayed no or opposite expression level changes when compared to control 10-day old kittens. These results therefore indicate that (i) visual experience is not necessary for the complete set of molecular events during primary visual cortex development, but (ii) binocular deprivation does alter the expression of a specific set of molecules, implicated in axon guidance and patterning.