Annual Meeting of the Society for Neuroscience edition:34 location:San Diego, CA, U.S.A. date:October 23-27, 2004
Collapsin Response Mediator Proteins (CRMP) are involved in neuronal differentiation through their regulation of growth cone collaps and axon guidance. In the visual cortex of normal cat, CRMPs exhibit a development-dependent expression, with high CRMP levels during the first postnatal weeks, and a clear decrease upon brain maturation. Semi-quantitative comparison of CRMP mRNA and protein expression levels, with real-time PCR in combination with laser microdissection (LMD) and Western blotting, between kitten (P30) and adult cat area 17 revealed a more pronounced decrease for CRMP4 compared to CRMP2 towards adulthood. In situ hybridisation and immunocytochemistry revealed the presence of CRMP2 and CRMP4 mRNA and protein in different cell types, including neurons and glial cells, and a typical lamination pattern for each CRMP. The role of CRMP2 and CRMP4 in developmental and adult cortical plasticity was investigated in monocular deprived kittens (P30) and adult cats with binocular retinal lesions. These visual deprivations had a significant effect on the expression of both CRMP proteins. We conclude that CRMP2 and CRMP4 show an age-related expression within cat area 17 and fulfil an important role during neuronal development under normal physiological conditions. Furthermore, they appear involved in plasticity-related processes not only during development but even throughout adulthood.