Title: Effect of ethylene glycol and glycerol fructosides on the activity and product specificity of bacterial and plant fructosyltransferases
Authors: Galonde, Nadine ×
Dyubankova, Natalia
Dongyan, Qin
Boutique, Jean-Pol
Lescrinier, Eveline
Van den Ende, Wim #
Issue Date: Dec-2010
Publisher: Harwood Academic Publishers
Series Title: Biocatalysis and Biotransformation vol:27 pages:328-339
Abstract: Fructosyltransferases (FTs) are key enzymes in plants and bacteria to synthesize fructans. To gain insight on the specificity of the hexose subsites in the active site of FTs, ethylene glycol fructoside (EGF) and glycerol fructoside (GF), containing fructose in the furanose configuration, were synthesized in vitro and used as substrates to study the effect on the activity of bacterial levansucrase (BsLS), chicory root sucrose:sucrose 1-fructosyltransferase (1-SST) and fructan:fructan 1-fructosyltransferase (1-FFT). The results demonstrated that EGF and GF, at physiologically relevant concentrations, were efficient acceptor substrates for BsLS and 1-FFT, but not for 1-SST. EGF and GF cannot be used as donor substrates for BsLS, 1-SST and 1-FFT. A model is proposed to explain the subsite specificity differences between the three FTs involved in this study.
ISSN: 1024-2422
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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