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Title: Quantification of chemokines by real-time reverse transcriptase PCR: applications in type 1 diabetes
Authors: Overbergh, Lutgart
Gysemans, Conny
Mathieu, Chantal # ×
Issue Date: Jan-2006
Publisher: Future Drugs Ltd
Series Title: Expert Review of Molecular Diagnostics vol:6 issue:1 pages:51-64
Abstract: Type 1 diabetes is a T-cell mediated autoimmune disease, characterized by the destruction of insulin-producing pancreatic beta-cells. This review will discuss the role of chemokines in the recruitment of immune cells leading to the pathology of this disease. There will be a focus on the quantification of chemokines and chemokine receptors by the recently developed real-time reverse transcriptase PCR technique. Today, this technique is in widespread use for analysis of chemokines in cells, tissues and tissue biopsies. The minute amount of tissue needed for analysis, as well as the very high sensitivity of this method, make it the method of choice for analysis of chemokines, which are often expressed at very low levels in target tissues. However, validation and optimization of the technique is of crucial importance for obtaining reliable results
URI: 
ISSN: 1473-7159
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical and Experimental Endocrinology
Medical Clerkships Centers
× corresponding author
# (joint) last author

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