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Title: Low-temperature switching by photoinduced protonation in photochromic fluorescent proteins
Authors: Regis Faro, Aline
Adam, Virgile
Carpentier, Philippe
Darnault, Claudine
Bourgeois, Dominique
de Rosny, Eve # ×
Issue Date: Feb-2010
Publisher: Royal Society of Chemistry
Series Title: Photochemical & Photobiological Sciences vol:9 issue:2 pages:254-262
Abstract: We have studied the photoswitching behaviour of a number of photochromic fluorescent proteins at cryo-temperature. Spectroscopic investigations at the ensemble level showed that EYFP, Dronpa and IrisFP all exhibit reversible photoswitching at 100 K, albeit with a low quantum yield. The photophysics of the process were studied in more details in the case of EYFP. The data suggest that photoinduced protonation of the chromophore is responsible for off-switching at cryo-temperature, and thus is possible in the absence of significant conformational freedom. This finding is consistent with the hypothesis that chromophore protonation may precede large amplitude conformational changes such as cis-trans isomerisation during off-photoswitching at room temperature. However, our data suggest that low-barrier photoinduced protonation pathways may in fact compete with room-temperature off-switching reactions in photochromic fluorescent proteins. The occurrence of reversible photoswitching at low-temperature is of interest to envisage cryonanoscopy experiments using genetically encoded fluorophores.
ISSN: 1474-905X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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