Author:

Keywords:

Animals, Brain, Electron Transport Complex IV, Enzyme Inhibitors, Hemoglobins, Hyperemia, Hypoxia, Brain, Kinetics, Male, NG-Nitroarginine Methyl Ester, Nitric Oxide, Nitric Oxide Synthase, Oxidation-Reduction, Oxygen, Oxyhemoglobins, Rats, Rats, Sprague-Dawley, Spectroscopy, Near-Infrared, Science & Technology, Life Sciences & Biomedicine, Endocrinology & Metabolism, Hematology, Neurosciences, Neurosciences & Neurology, nitric oxide, near-infrared spectroscopy, L-NAME, cytochrome oxidase, brain, rat, NEAR-INFRARED SPECTROSCOPY, OXYGEN-CONSUMPTION, BLOOD-FLOW, SYNTHASE INHIBITION, RESPIRATORY-CHAIN, ARTERY OCCLUSION, C-OXIDASE, BRAIN, HYPOTHERMIA, INVIVO, 1102 Cardiorespiratory Medicine and Haematology, 1103 Clinical Sciences, 1109 Neurosciences, Neurology & Neurosurgery, 3202 Clinical sciences, 3209 Neurosciences

Abstract:

In this study, near-infrared spectroscopy was applied to examine whether cytochrome oxidase in the rat brain is inhibited by nitric oxide in vivo. During normoxia, intravenous N(G)-nitro-L-arginine methyl ester (L-NAME) administration significantly decreased the cerebral saturation of hemoglobin with oxygen but did not alter the cytochrome oxidase redox state. Anoxia significantly reduced the cytochrome oxidase. The time course of the recovery of the redox state during reoxygenation was not altered by L-NAME. The results suggest that in adult rats, cytochrome oxidase is not inhibited by nitric oxide, either in physiologic conditions or during reoxygenation after a brief anoxic period.