Acta Neurologica Belgica. Program and Abstract Book. pages:29
Bi-Annual Meeting of the Belgian Society for Neuroscience edition:8 location:Liège date:11 May 2009
Matrix assisted laser desorption/ionisation imaging mass spectrometry (MALDI-IMS) is an emerging technology to map molecules of interest in thin tissue sections. The power of IMS resides in the label-free detection of the complete proteome of a tissue slice. In combination with hierarchical clustering and principal component analysis (PCA), IMS makes an accessible and powerful tool for candidate biomarker screening. In this study, coronal mouse brain sections (C57Bl6J) have been screened for candidate biomarkers capable of discriminating anatomical subdivisions in the brain. Following clustering and PCA, a number m/z-values could be appointed as region-specific markers for the hippocampus, cortex, white matter and thalamus. This study underlines the value of MALDI IMS and subsequent data analysis to discover new regional biomarkers.