Title: Discovery of brain region-specific protein expression via imaging mass spectrometry: proof-of-principle
Authors: Minerva, Laurens ×
Dierckx, S.
Arckens, Lut #
Issue Date: 11-May-2009
Host Document: Acta Neurologica Belgica. Program and Abstract Book. pages:29
Conference: Bi-Annual Meeting of the Belgian Society for Neuroscience edition:8 location:Liège date:11 May 2009
Abstract: Matrix assisted laser desorption/ionisation imaging mass spectrometry (MALDI-IMS) is an emerging technology to map molecules of interest in thin tissue sections. The power of IMS resides in the label-free detection of the complete proteome of a tissue slice. In combination with hierarchical clustering and principal component analysis (PCA), IMS makes an accessible and powerful tool for candidate biomarker screening. In this study, coronal mouse brain sections (C57Bl6J) have been screened for candidate biomarkers capable of discriminating anatomical subdivisions in the brain. Following clustering and PCA, a number m/z-values could be appointed as region-specific markers for the hippocampus, cortex, white matter and thalamus. This study underlines the value of MALDI IMS and subsequent data analysis to discover new regional biomarkers.
Publication status: published
KU Leuven publication type: AMa
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.


All items in Lirias are protected by copyright, with all rights reserved.