Author:

Keywords:

1-Methyl-3-isobutylxanthine, 3',5'-Cyclic-GMP Phosphodiesterase, Calcium, Calmodulin, Cyclic GMP, Erythrocytes, Humans, Hydrolysis, Phosphodiesterase Inhibitors, Purinones, Sulfonamides, Vinca Alkaloids, Science & Technology, Life Sciences & Biomedicine, Pharmacology & Pharmacy, erythrocytes, cyclic nucleotide phosphodiesterase, phosphodiesterase inhibitors, Ca2+, calmodulin-dependent phosphodiesterase, calmodulin antagonists, PROTEIN-KINASE-C, GUANYLATE-CYCLASE, SMOOTH-MUSCLE, GMP LEVELS, BOVINE, STIMULATION, CELLS, ACCUMULATION, INHIBITION, ACTIVATION, 3',5'-Cyclic-GMP Phosphodiesterases, 1115 Pharmacology and Pharmaceutical Sciences, 3214 Pharmacology and pharmaceutical sciences

Abstract:

To determine whether phosphodiesterase (PDE) is involved in the degradation of cGMP in human erythrocytes, we studied the cell cGMP content in the presence of different PDE inhibitors: zaprinast and dipyridamole, specific inhibitors of cGMP-binding, cGMP-specific PDE (cG-BPDE); vinpocetine, a specific inhibitor of Ca2+, calmodulin-dependent phosphodiesterase (CaM-PDE); an unspecific inhibitor, 3-isobutyl-1-methylxanthine (IBMX). IBMX, zaprinast, and dipyridamole at 30 microM did not affect the intracellular cGMP content. However, vinpocetine at this concentration increased the cGMP content by 102 +/- 14% (p < 0.05). The effect of vinpocetine was dose-dependent, reached the maximal level after 1 min of incubation and flattened at the same level. Ca2+ (10 microM) in the presence of the Ca(2+)-ionophore, A23187 (5 microM), decreased the cGMP content (-23% +/- 4; p < 0.05), which can be explained by the CaM-PDE activation. The Ca(2+)-induced decrease in cGMP was completely inhibited by the CaM antagonist, W-7 (100 microM). These data suggest that erythrocytes contain Ca2+, CaM-PDE.