Title: Peptide hormone immunocytochemistry in insects
Authors: Verhaert, P. ×
Schols, D.
Vanden Broeck, Jozef
De Loof, Arnold #
Issue Date: 1987
Publisher: Chapman and Hall
Series Title: The Histochemical Journal vol:19 pages:309
Conference: Immunocytochemistry Club Meeting on In Situ Hybridization Histochemistry location:London date:March 1987
Article number: -
Abstract: Using a number of specific polyclonal and monoclonal
(Mab) antisera antibodies to vertebrate peptide hormones
related to the gastro-entero-pancreatic system,
positive immunohistochemical results were obtained
in the American cockroach, Periplaneta americana (L.).
Neurosecretory neuron-like cells and their processes
in the brain and/or nerve terminals in the corpus
cardiacum (an important insect neurohaemal organ)
were observed to be selectively labelled by antibodies
to vasoactive intestinal polypeptide and peptide histidine
isoleucine 1 (labelling distinct materials), gastrin
and cholecystokinin 2 (also resulting in a different
distribution of the immunoreaction), bombesin/gastrin
releasing peptide I (two different antisera giving
entirely different staining patterns), neurotensin 3,
insulin-like growth factor I (Mab), insulin, glucagon 3,
somatostatin, pancreatic polypeptide and substance P
(Mab), the latter two being published earlier [Verhaert
et al., Brain Res. 348, 331-8 (1985); Verhaert & De Loof,
Histochemistry 83, 501-7 (1985), respectively]. Moreover,
except for the PHI antiserum, which only stained
some intestinal nerves, antibodies to all these hormones
specifically marked both cells of the paraneuron
(endocrine) type in the midgut wall and nerve
fibres terminating on the alimentary epithelium.
These data are in favour of the theory that in insects as
well as in vertebrates, peptide-like chemicals may exist
having a dual localization, i.e. centrally, in the brain as
well as peripherally, in the gut. Preliminary experiments
using radiolabelled vertebrate glucagon and
insulin cDNAs in in situ hybrization histochemistry
indicate that one has to be very cautious in interpreting
(RNA dependent) hybridization results obtained
with heterologous probes. A general (non-specific?)
distribution of radiolabelling was observed in the
cockroach brain cells, in identical conditions of stringency
(i.e. 50% formamide, 37°C, 2xSSC) which allow a
(specific) marking of the relevant cells of vertebrate
pancreatic tissue.
ISSN: 0018-2214
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.