Methods and findings in experimental and clinical pharmacology vol:11 issue:9 pages:587-94
The effects of xipamide on the intracellular concentration and transmembrane fluxes of Na+ and K+ were studied in twenty-four normal sodium-replete or -deplete male subjects, using a double-blind study design. After a run-in period on placebo for 1 week on their regular diet, subjects were treated on their regular diet or on a low-sodium diet with either placebo or xipamide 20 mg once a day for 16 weeks. Intra-erythrocyte Na+ concentration was increased during xipamide administration in sodium-replete and deplete subjects, while intra-erythrocyte K+ and total intraleukocyte Ca2+ concentrations were decreased. Red cell Na+, K+-cotransport activity was lower in xipamide-treated subjects, while Na+, Li-countertransport activity was increased. No significant effect of xipamide could be demonstrated on ouabain-sensitive 86Rb-uptake or on maximal [3H]-ouabain binding in erythrocytes.