Title: Characterization of Kafirins in Algerian Sorghum Cultivars
Authors: Mokrane, Hind ×
Lagrain, Bert
Gebruers, Kurt
Courtin, Christophe
Brijs, Kristof
Proost, Paul
Delcour, Jan #
Issue Date: Oct-2009
Publisher: Amer assoc cereal chemists
Series Title: Cereal chemistry vol:86 issue:5 pages:487-491
Abstract: The prolamins in seven Algerian Sahara sorghum cultivars of varying seed shape and color were investigated. Protein contents ranged from 12 to 16%. Prolamins were the major protein fraction. They could be separated according to degree of disulfide cross-linking. Kafirin monomers and low molecular weight polymers could be extracted with 70% ethanol, whereas highly cross-linked kafirins additionally needed a reducing agent to become extractable. Kafirin monomers of alpha-, beta- and gamma-type were purified and N-terminally sequenced. For the first time, delta-kafirin was identified at the protein level. The study clearly revealed intercultivar differences between protein levels. The joint use of SDS-PAGE, SE-HPLC, and RP-HPLC allowed discriminating among cultivars based on the differences in prolamin levels and composition.
ISSN: 0009-0352
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Laboratory of Molecular Immunology (Rega Institute)
× corresponding author
# (joint) last author

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