Title: A Plasma-Based Biomatrix Mixed with Endothelial Progenitor Cells and Keratinocytes Promotes Matrix Formation, Angiogenesis, and Reepithelialization in Full-Thickness Wounds
Authors: Vermeulen, Pieter ×
Dickens, Stijn
Degezelle, Karlien
Van den Berge, Stefaan
Hendrickx, Benoit
Vranckx, Jan #
Issue Date: Jul-2009
Publisher: Mary Ann Liebert Inc
Series Title: Tissue Engineering Part A vol:15 issue:7 pages:1533-1542
Abstract: In search of an autologous vascularized skin substitute, we treated full-thickness wounds (FTWs) with autologous platelet-rich plasma gel (APG) in which we embedded endothelial progenitor cells (EPCs) and basal cell keratinocytes (KCs). We cultivated autologous KCs in low-serum conditions and expanded autologous EPCs from venous blood. FTWs (n = 55) were created on the backs of four pigs, covered with wound chambers, and randomly assigned to the following treatments: (1) APG, (2) APG+KCs, (3) APG+EPCs, (4) APG+KCs+EPCs, and (5) saline. All wounds were biopsied to measure neovascularization (lectin Bandeiraea Simplicifolia-1 (BS-1), alpha smooth muscle actin [alpha SMA], and membrane type 1 matrix metalloproteinase (MT1-MMP)), matrix deposition (fibronectin, collagen type I/III, and alpha v beta 3), and reepithelialization. Wound fluids were analyzed for protein expression. All APG-treated wounds showed more vascular structures (p< 0.001), and the addition of EPCs further improved neovascularization, as confirmed by higher lectin, alpha SMA, and MT1-MMP. APG groups had higher collagen I/III (p< 0.05), alpha v beta 3, and fibronectin content (p< 0.001), and they exhibited higher concentrations of platelet-derived growth factor subunit bb, basic fibroblast growth factor, hepatocyte growth factor, insulin growth factor-1, transforming growth factor-beta 1 and -beta 3, matrix metalloproteinase-1 and -z9, and tissue-inhibiting matrix metalloproteinase-1 and -2. Applying APG+KCs resulted in the highest reepithelialization rates (p< 0.001). No differences were found for wound contraction by planimetry. In this porcine FTW model, APG acts as a supportive biomatrix that, along with the embedded cells, improves extracellular matrix organization, promotes angiogenesis, and accelerates reepithelialization.
ISSN: 1937-3341
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Plastic, Reconstructive and Estetic Surgery Section (-)
× corresponding author
# (joint) last author

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