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Title: Helicobacter pylori cag pathogenicity island-positive strains induce syndecan-4 expression in gastric epithelial cells
Authors: Magalhaes, Ana ×
Marcos, Nuno T
Carvalho, Ana S
David, Leonor
Figueiredo, Ceu
Bastos, Joana
David, Guido
Reis, Celso A #
Issue Date: Aug-2009
Publisher: Elsevier Science Publishers, B.V. on behalf of the Federation of European Microbiological Societies
Series Title: FEMS Immunology and Medical Microbiology vol:56 issue:3 pages:223-232
Abstract: Helicobacter pylori is recognized as the main cause of gastritis and is associated with gastric carcinogenesis. Syndecan-4 represents the major source of heparan sulfate (HS) in the gastric cells. HS proteoglycans expressed on the cell surface constitute targets for H. pylori at the early stage of infection. The aim of this study was to determine whether H. pylori induction of syndecan-4 expression is affected by the virulence characteristics of the infecting strain, namely the cytotoxic-associated gene (cag) pathogenicity island (PAI). We observed that individuals infected with highly pathogenic H. pylori strains express syndecan-4 in the foveolar epithelium of the gastric mucosa. The association between the cagPAI status of the infecting strain and syndecan-4 expression was further demonstrated by infection of gastric epithelial cell lines with a panel of cagPAI(+) and cagPAI(-) H. pylori strains, showing that expression of syndecan-4 was significantly increased in response to infection with the highly pathogenic strains. Moreover, infection of gastric cells with cagA and cagE mutant strains further confirmed that syndecan-4 induction is dependent on an intact cagPAI. The present study shows that highly pathogenic H. pylori strains induce syndecan-4 expression, both in human gastric mucosa and in gastric cell lines, in a cagPAI-dependent manner.
URI: 
ISSN: 0928-8244
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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