Title: A high-performance liquid chromatography radio method for determination of L-ascorbic acid and guanosine 5 '-diphosphate-L-galactose, key metabolites of the plant vitamin C pathway
Authors: Wolucka, BA ×
Davey, Mark
Boerjan, W #
Issue Date: Jul-2001
Publisher: Academic press inc
Series Title: Analytical biochemistry vol:294 issue:2 pages:161-168
Abstract: A simple, rapid, and quantitative high-pressure liquid chromatography radio method is described for the determination of in vivo C-14-labeled L-ascorbate, dehydro-L-ascorbate, and total L-ascorbate of Arabidopsis thaliana cell suspensions upon incubation of cultures with exogenous D-[C-14]mannose. The same radio-HPLC conditions can be used to follow the products of in vitro enzymatic conversions of GDP-D-mannose by enzyme extracts of A thaliana, namely GDP-L-galactose, GDP-4 " -keto,6 " -deoxy-D-mannose, and GDP-L-fucose. In particular, an accurate assay for GDP-D-mannose 3 " ,5 " -epimerase, a key enzyme of the plant vitamin C pathway, is presented. (C) 2001 Academic Press.
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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