Ascorbate biosynthesis in Arabidopsis cell suspension culture
Davey, Mark × Gilot, C Persiau, G Ostergaard, J Han, Y Bauw, GC Van Montagu, MC #
Amer soc plant physiologists
Plant physiology vol:121 issue:2 pages:535-543
The biosynthesis of L-ascorbic acid (L-AA) in an Arabidopsis (L.) Heynh. cell suspension culture was studied by quantifying the effects of incubation with a range of potential biosynthetic precursors, analogs, and inhibitors on the intracellular levels of reduced and oxidized forms of L-AA. Our results support the recently published biosynthetic pathway of L-AA from L-galactose (G.L. Wheeler, M.A. Jones, N. Smirnoff  Nature 393: 365-369), but suggest that Arabidopsis cell suspension culture simultaneously contains two other routes leading to L-AA. The possible physiological significance of these alternate routes is discussed.