Title: Analysis of ascorbate in plant tissues by high-performance capillary zone electrophoresis
Authors: Davey, Mark ×
Bauw, G
VanMontagu, M #
Issue Date: Jul-1996
Publisher: Academic press inc jnl-comp subscriptions
Series Title: Analytical biochemistry vol:239 issue:1 pages:8-19
Abstract: We describe here a simple and rapid capillary electrophoresis method for the determination of ascorbic acid (L-AA) and isoascorbic acid (D-AA) in vegetative tissues. For optimal yields and stabilization, samples are extracted with cold 3% metaphosphoric acid. Hydrophobic contaminants are then removed by passage through a C-18 solid-phase extraction cartridge. The analysis itself is performed on a fused silica capillary with 200 mM berate, pH 9, as the carrier electrolyte, using on-line diode array detection over the range 190-350 nm. Quantitation was performed at 260 nm, the uv-absorption maximum for ascorbate at this pH. This method has a minimum detection Limit of 84 fmol/injection and linearity of detector response was observed up to at least 12 pmol/injection. We also describe the influence of electrolyte concentration, pH, and the presence of detergent on separations of L-AA, D-AA, and L-galacturonic acid-1,4-lactone. The protocol has been demonstrated to be suitable for the analysis of L-AA in Arabidopsis, parsley, and mushroom. The method has superior resolution to comparable HPLC separations, a comparable analysis time, but lower sensitivity because of the concentration limitations of the detection system. (C) 1996 Academic Press, Inc.
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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