Title: Pharmacokinetics and coronary thrombolytic properties of two human tissue-type plasminogen activator variants lacking the finger-like, growth factor-like, and first kringle domains (amino acids 6-173) in a canine model
Authors: Wu, Z ×
Van de Werf, Frans
Stassen, Antoni Maria
Mattsson, C
Pohl, G
Collen, Desire #
Issue Date: Aug-1990
Series Title: Journal of cardiovascular pharmacology vol:16 issue:2 pages:197-203
Abstract: The pharmacokinetics and thrombolytic properties of two variants of recombinant human tissue-type plasminogen activator (rt-PA) were studied in dogs with a copper coil induced thrombosis of the left anterior descending coronary artery. The first variant, rt-PA-delta FEK1-Gln184, lacked amino acids 6 to 173 [comprising the fibronectin finger-like (F), the epidermal growth factor-like (E), and the first kringle (K1) domains] and had the glycosylated Asn184 mutagenized to Gln. The second variant, rt-PA-delta FEK1-Gln184, Val277, had in addition Lys277 mutagenized to Val. Injection of 0.25, 0.50, or 1.0 mg/kg of rt-PA in groups of three dogs caused reflow in six of nine dogs, within 18 +/- 15 min (mean +/- SD), but was associated with reocclusion within 2 h in all animals. Injection of 0.125, 0.25, or 0.50 mg/kg of rt-PA-delta FEK1-Gln184 caused reflow in all of nine dogs, within 17 +/- 23 min, with persistent patency in four animals (p = 0.02 vs. rt-PA). Bolus injection of 4:1 mixtures of rt-PA-delta FEK1-Gln184 and rt-PA in total amounts of 0.125, 0.25, or 0.50 mg/kg resulted in reflow in eight of nine dogs within 25 +/- 21 min with persistent patency in seven (p = 0.003 vs. rt-PA, p = 0.25 vs. rt-PA-delta FEK1-Gln184 alone). Injection of 0.25, 0.50, or 1.0 mg/kg of rt-PA-delta FEK1-Gln184, Val277 produced reperfusion in six of nine dogs, within 27 +/- 26 min, with persistent patency in three (p = 0.59 vs. rt-PA and p = 0.23 vs. rt-PA-delta FEK1-Gln184).(ABSTRACT TRUNCATED AT 250 WORDS)
ISSN: 0160-2446
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Cardiology
Molecular and Vascular Biology
Experimental Cardiology
× corresponding author
# (joint) last author

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