Title: Androgen receptor disruption increases the osteogenic response to mechanical loading in male mice
Authors: Callewaert, Filip ×
Bakker, Astrid
Schrooten, Jan
Van Meerbeek, Bart
Verhoeven, Guido
Boonen, Steven
Vanderschueren, Dirk #
Issue Date: Jan-2010
Publisher: Blackwell Science, Inc.
Series Title: Journal of Bone and Mineral Research vol:25 issue:1 pages:124-131
Abstract: Abstract In female mice, estrogen receptor-alpha (ERalpha) mediates the anabolic response of bone to mechanical loading. Whether ERalpha plays a similar role in the male skeleton, and to what extent androgens and androgen receptor (AR) affect this response in males remains unaddressed. Therefore, we studied the adaptive response of in vivo ulna loading in AR-ERalpha knockout (KO) mice and corresponding male and female single KO and wild-type (WT) littermates, using dynamic histomorphometry and immunohistochemistry. Additionally, cultured bone cells from WT and ARKO mice were subjected to mechanical loading by pulsating fluid flow in the presence or absence of testosterone. In contrast with female mice, ERalpha inactivation in male mice had no effect on the response to loading. Interestingly, loading induced significantly more periosteal bone formation in ARKO (+320%) and AR-ERalphaKO mice (+256%) compared to male WT mice (+114%), and had a stronger inhibitory effect on SOST/sclerostin expression in ARKO vs. WT mice. In accordance, the fluid flow-induced nitric oxide production was higher in the absence of testosterone in bone cells from WT, but not ARKO mice. In conclusion, AR, but not ERalpha activation limits the osteogenic response to loading in male mice, possibly via an effect on Wnt signaling.
ISSN: 0884-0431
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biomaterials - BIOMAT
Physical Metallurgy and Materials Engineering Section (-)
Department of Materials Engineering - miscellaneous
Clinical and Experimental Endocrinology
Gerontology and Geriatrics
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
pub05307.pdfMain article Published 241KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science