3,5-Bis(benzylidene)-4-oxo-1-phosphonopiperidines and related diethyl esters: potent cytotoxins with multi-drug-resistance reverting properties
Das, Swagatika × Das, Umashankar Selvakumar, Ponniah Sharma, Rajendra K Balzarini, Jan De Clercq, Erik Molnár, Joseph Serly, Julianna Baráth, Zoltán Schatte, Gabriele Bandy, Brian Gorecki, Dennis K J Dimmock, Jonathan R #
Wiley - V C H Verlag GmbH & Co. KGaA
ChemMedChem vol:4 issue:11 pages:1831-1840
A series of 3,5-bis(benzylidene)-4-piperidones 3 were converted into the corresponding 3,5-bis(benzylidene)-1-phosphono-4-piperidones 5 via diethyl esters 4. The analogues in series 4 and 5 displayed marked growth inhibitory properties toward human Molt 4/C8 and CEM T-lymphocytes as well as murine leukemia L1210 cells. In general, the N-phosphono compounds 5, which are more hydrophilic than the analogues in series 3 and 4, were the most potent cluster of cytotoxins, and, in particular, 3,5-bis-(2-nitrobenzylidene)-1-phosphono-4-piperidone 5 g had an average IC(50) value of 34 nM toward the two T-lymphocyte cell lines. Four of the compounds displayed potent cytotoxicity toward a panel of nearly 60 human tumor cell lines, and nanomolar IC(50) values were observed in a number of cases. The mode of action of 5 g includes the induction of apoptosis and inhibition of cellular respiration. Most of the members of series 4 as well as several analogues in series 5 are potent multi-drug resistance (MDR) reverting compounds. Various correlations were noted between certain molecular features of series 4 and 5 and cytotoxic properties, affording some guidelines in expanding this study.