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Title: Conditional mutagenesis in Drosophila
Authors: Choi, Ching-Man *
Vilain, Sven *
Langen, Marion
Van Kelst, Sofie
De Geest, Natalie
Yan, Jiekun
Verstreken, Patrik
Hassan, Bassem # ×
Issue Date: Apr-2009
Publisher: American Association for the Advancement of Science
Series Title: Science vol:324 issue:5923 pages:54
Abstract: Most genes function at multiple stages of metazoan development, in dividing and nondividing cells. Generating mouse conditional knock-outs (cKO), where a gene can be eliminated in a temporally and spatially controlled manner, is a valuable technique because it allows study of gene function at any stage of life. In contrast and despite the development of many other powerful genetic tools, cKO has thus far been lacking in Drosophila. We combined several recent molecular and genetic technical advances in an approach termed integrase-mediated approach for gene knock-out (IMAGO). IMAGO allows the replacement of any genomic sequence, such as a gene, with another desired sequence, including cKO alleles that can be used to create positively marked mutant cells. IMAGO should also be applicable to other genetic model organisms.
ISSN: 0036-8075
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Molecular Genetics Section (-)
Laboratory of Neuronal Communication
Laboratory of Neurogenetics
* (joint) first author
× corresponding author
# (joint) last author

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