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Title: Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake
Authors: Kasprowicz, Jaroslaw *
Kuenen, Sabine *
Miskiewicz, Katarzyna
Habets, Ron L P
Smitz, Liesbet
Verstreken, Patrik # ×
Issue Date: Sep-2008
Publisher: Rockefeller Institute Press
Series Title: Journal of Cell Biology vol:182 issue:5 pages:1007-1016
Abstract: Synaptic vesicle reformation depends on clathrin, an abundant protein that polymerizes around newly forming vesicles. However, how clathrin is involved in synaptic recycling in vivo remains unresolved. We test clathrin function during synaptic endocytosis using clathrin heavy chain (chc) mutants combined with chc photoinactivation to circumvent early embryonic lethality associated with chc mutations in multicellular organisms. Acute inactivation of chc at stimulated synapses leads to substantial membrane internalization visualized by live dye uptake and electron microscopy. However, chc-inactivated membrane cannot recycle and participate in vesicle release, resulting in a dramatic defect in neurotransmission maintenance during intense synaptic activity. Furthermore, inactivation of chc in the context of other endocytic mutations results in membrane uptake. Our data not only indicate that chc is critical for synaptic vesicle recycling but they also show that in the absence of the protein, bulk retrieval mediates massive synaptic membrane internalization.
ISSN: 0021-9525
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Laboratory of Neuronal Communication
* (joint) first author
× corresponding author
# (joint) last author

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