Title: Dendritic LSm1/CBP80-mRNPs mark the early steps of transport commitment and translational control
Authors: di Penta, Alessandra
Mercaldo, Valentina
Florenzano, Fulvio
Munck, Sebastian
Zalfa, Francesca
Ciotti, M. Teresa
Mercanti, Delio
Molinari, Marco
Bagni, Claudia
Achsel, Tilmann # ×
Issue Date: Feb-2009
Publisher: Rockefeller Institute Press
Series Title: Journal of Cell Biology vol:184 issue:3 pages:423-35
Abstract: Messenger RNA (mRNA) transport to neuronal dendrites is crucial for synaptic plasticity, but little is known of assembly or translational regulation of dendritic messenger ribonucleoproteins (mRNPs). Here we characterize a novel mRNP complex that is found in neuronal dendrites throughout the central nervous system and in some axonal processes of the spinal cord. The complex is characterized by the LSm1 protein, which so far has been implicated in mRNA degradation in non-neuronal cells. In brain, it associates with intact mRNAs. Interestingly, the LSm1-mRNPs contain the cap-binding protein CBP80 that associates with (pre) mRNAs in the nucleus, suggesting that the dendritic LSm1 complex has been assembled in the nucleus. In support of this notion, neuronal LSm1 is partially nuclear and inhibition of mRNA synthesis increases its nuclear localization. Importantly, CBP80 is also present in the dendrites and both LSm1 and CBP80 shift significantly into the spines upon stimulation of glutamergic receptors, suggesting that these mRNPs are translationally activated and contribute to the regulated local protein synthesis.
ISSN: 0021-9525
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Laboratory for the Research of Neurodegenerative Diseases
Molecular Genetics Section (-)
× corresponding author
# (joint) last author

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