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Keywords:

amino acid, amino-acids, gc-ms, humans, mass-spectrometry, stable isotope, Science & Technology, Physical Sciences, Chemistry, Analytical, Chemistry, GC-MS, MASS-SPECTROMETRY, AMINO-ACIDS, HUMANS, 0301 Analytical Chemistry, 0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology, Analytical Chemistry, 3101 Biochemistry and cell biology, 3401 Analytical chemistry

Abstract:

In order to study protein digestibility by means of noninvasive tracer techniques (stable isotopes), a representative oral tracer, Le. a stable isotope labeled protein, is needed. Therefore, egg white containing L-[ring-H-2(5)]phenylalanine and L-[ring-H-2(4)]tyrosine was prepared. The aim of this study was to measure the isotopic enrichment of the labeled amino acids in the egg white. The use of a standard GC-MS, based on ion trap technology was found to be a reliable technique. The enrichment of L-[ring-H-2(4)]phenylalanine and L-[ring-H-2(4)]tyrosine, expressed in Molar Percent (MP) amounted to 23.2 MP and 2.8 MP respectively.