Detection of antisynthetic mannoside antibodies (A Sigma MA) reveals heterogeneity in the ASCA response of Crohn's disease patients and contributes to differential diagnosis, stratification, and prediction
Khoury, Peggy Vandewalle-El × Colombel, Jean-Frederic Joossens, Sofie Standaert-Vitse, Annie Collot, Mayeul Halfvarson, Jonas Ayadi, Ali Landers, Carol J Vermeire, Severine Rutgeerts, Paul Targan, Stephan R Chamaillard, Mathias Mallet, Jean-Maurice Sendid, Boualem Poulain, Daniel #
Nature Publishing Group
American Journal of Gastroenterology vol:103 issue:4 pages:949-957
OBJECTIVES: Anti-S. cerevisiae mannan antibodies (ASCA) are human antibodies associated with Crohn's disease (CD) reacting with Saccharomyces cerevisiae (S. cerevisiae) mannan polymer. As mannan is a complex and variable repertoire of oligomannoses acting as epitopes, we chemically synthesized (Sigma) two major oligomannose epitopes, Man alpha-1,3 Man alpha-1,2 Man (Sigma Man3) and Man alpha-1,3 Man alpha-1,2 Man alpha-1,2 Man (Sigma Man4), and then explored how antisynthetic mannoside antibodies (A Sigma MA) compare with ASCA as markers of CD.