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Title: The absence of thrombospondin-2 causes age-related dilated cardiomyopathy
Authors: Swinnen, Melissa ×
Vanhoutte, Davy
Van Almen, G
Hamdani, N
Schellings, M.W.M
D'hooge, Jan
Van der Velden, J
Weaver, M.S
Sage, E.H.
Bornstein, P
Verheyen, F.K.
Vandendriessche, Thierry
Chuah, Marinee
Westernann, D
Paulus, W.J.
Van de Werf, Frans
Schroen, Blanche
Carmeliet, Peter
Pinto, YM
Heymans, S #
Issue Date: 20-Oct-2009
Publisher: American Heart Association
Series Title: Circulation vol:120 issue:16 pages:1585-1597
Abstract: BACKGROUND: The progressive shift from a young to an aged heart is characterized by alterations in the cardiac matrix. The present study investigated whether the matricellular protein thrombospondin-2 (TSP-2) may affect cardiac dimensions and function with physiological aging of the heart. METHODS AND RESULTS: TSP-2 knockout (KO) and wild-type mice were followed up to an age of 60 weeks. Survival rate, cardiac function, and morphology did not differ at a young age in TSP-2 KO compared with wild-type mice. However, >55% of the TSP-2 KO mice died between 24 and 60 weeks of age, whereas <10% of the wild-type mice died. In the absence of TSP-2, older mice displayed a severe dilated cardiomyopathy with impaired systolic function, increased cardiac dilatation, and fibrosis. Ultrastructural analysis revealed progressive myocyte stress and death, accompanied by an inflammatory response and replacement fibrosis, in aging TSP-2 KO animals, whereas capillary or coronary morphology or density was not affected. Importantly, adeno-associated virus-9 gene-mediated transfer of TSP-2 in 7-week-old TSP-2 KO mice normalized their survival and prevented dilated cardiomyopathy. In TSP-2 KO animals, age-related cardiomyopathy was accompanied by increased matrix metalloproteinase-2 and decreased tissue transglutaminase-2 activity, together with impaired collagen cross-linking. At the cardiomyocyte level, TSP-2 deficiency in vivo and its knockdown in vitro decreased the activation of the Akt survival pathway in cardiomyocytes. CONCLUSIONS: TSP-2 expression in the heart protects against age-dependent dilated cardiomyopathy.
ISSN: 0009-7322
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Cardiovascular Imaging and Dynamics
Vesalius Research Centre (-)
Cardiology
Molecular and Vascular Biology
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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