Title: Cerebral blood flow assessment with indocyanine green bolus transit detection by near-infrared spectroscopy in the rat
Authors: De Visscher, Geofrey ×
van Rossem, Koen
Van Reempts, Jos
Borgers, Marcel
Flameng, Willem
Reneman, Robert S #
Issue Date: May-2002
Series Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology vol:132 issue:1 pages:87-95
Abstract: In this study we evaluated the feasibility of measuring cerebral blood flow in rats by monitoring the transit of an indocyanine green bolus through the brain with multiwavelength near-infrared spectroscopy. Different volumes of a 1 mg/ml indocyanine green solution (5, 15, 25, 50 microl) were injected intravenously in the search for an optimal dose. Clear transit curves were obtained with all doses and a blood flow index could easily be determined. The indocyanine green signal obtained with the bolus of 5 microl rapidly returned to baseline and interfered minimally with the haemoglobin and cytochrome oxidase signals. This dose was used in a second study to evaluate the reproducibility of the signal and the effect of hypercapnia. Two groups of rats received 7 repetitive boli of indocyanine green. In one group, 7% CO(2) was added to the gas mixture before the second, fourth and sixth indocyanine green injection. Hypercapnia consistently caused a significant increase in blood flow index, cerebral haemoglobin concentration and O(2)-saturation. In the control group these variables remained stable in time. We conclude that monitoring of the transit of an indocyanine green bolus with multiwavelength near-infrared spectroscopy can be used to assess cerebral blood flow qualitatively in rats in combination with continuous monitoring of brain oxygenation.
ISSN: 1095-6433
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Experimental Cardiac Surgery (-)
× corresponding author
# (joint) last author

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