Title: Validation of a microchip electrophoresis system as a DNA amplification control
Authors: Beakaert, Bram ×
Commans, Monique
Knaepen, Katleen
Larno, Lucie
Thijs, Nathalie
Vanhoutte, Elisabeth
Van de Voorde, Wim
Decorte, Ronny #
Issue Date: 2009
Publisher: Elsevier
Host Document: Forensic Science International. Genetics Supplement Series vol:2 pages:119-120
Conference: 23rd World Congress of the International Society for Forensic Genetics location:Buenos Aires (Argentina) date:15-18 September 2009
Abstract: As part of the normal procedure in a forensic DNA laboratory, a quality control step of the
amplified DNA is often implemented to ensure the correct amplification of the sample before it is
analysed in downstream applications. A validation study was undertaken to investigate a new
microchip electrophoresis system (MultiNa, Shimadzu Corporation) claiming high resolution and
sensitivity compared to routine polyacrylamide gel electrophoresis (PAGE). An array of STR
multiplexes (AmpFISTR™ SGM+, GenePrint® FFFL, PowerPlex™ 16, PowerPlex™ Y, an in-house Y-STR
multiplex and AmpFISTR™ Profiler) were tested under both standard and low copy number PCR
parameters to evaluate the accuracy, reproducibility and sensitivity of this technique. These tests
showed that the microchip system did not have improved sensitivity compared to PAGE though had
increased resolution and high reproducibility between samples.
ISSN: 1875-1768
Publication status: published
KU Leuven publication type: IC
Appears in Collections:Department of Human Genetics - miscellaneous
Forensic Biomedical Sciences
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.