Forensic Science International. Genetics Supplement Series vol:2 pages:123-124
23rd World Congress of the International Society for Forensic Genetics location:Buenos Aires (Argentina) date:15-18 September 2009
Free fluorescent dyes from PCR primers or amplification products can interfere with the interpretation of STR alleles in an electropherograph especially when the profiles have a low signal intensity. These artefacts can be removed by using a simple procedure based on BigDye® XTerminator™. This procedure requires limited amounts of PCR product, allows to do several loadings on a capillary sequencer starting from the same purified PCR product and also increases the sensitivity for detection of less amplified loci.