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Title: Increased sensitivity for amplified STR alleles on capillary sequencers with BigDye® XTerminator™
Authors: Janssen, Lynn
Jacobs, Manon
Vanderheyden, Nancy
Bekaert, Bram
Van de Voorde, Wim
Decorte, Ronny # ×
Issue Date: 2009
Publisher: Elsevier
Host Document: Forensic Science International. Genetics Supplement Series vol:2 pages:123-124
Conference: 23rd World Congress of the International Society for Forensic Genetics location:Buenos Aires (Argentina) date:15-18 September 2009
Abstract: Free fluorescent dyes from PCR primers or amplification products can interfere with the interpretation of STR alleles in an electropherograph especially when the profiles have a low signal intensity. These artefacts can be removed by using a simple procedure based on BigDye® XTerminator™. This procedure requires limited amounts of PCR product, allows to do several loadings on a capillary sequencer starting from the same purified PCR product and also increases the sensitivity for detection of less amplified loci.
ISSN: 1875-1768
Publication status: published
KU Leuven publication type: IC
Appears in Collections:Department of Human Genetics - miscellaneous
Forensic Biomedical Sciences
× corresponding author
# (joint) last author

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