Title: Rolling-circle amplification of viral DNA genomes using phi29 polymerase
Authors: Johne, Reimar ×
Mueller, Hermann
Rector, Annabel
Van Ranst, Marc
Stevens, Hans #
Issue Date: May-2009
Publisher: Elsevier science london
Series Title: Trends in Microbiology vol:17 issue:5 pages:205-211
Abstract: Techniques for the single-step amplification of whole genomes have been developed into powerful tools for phylogenetic analyses, epidemiological studies and studies on genome organization. Recently, the bacteriophage phi29 DNA polymerase has been used for the efficient amplification of circular DNA viral genomes without the need of specific primers by a rolling-circle amplification (RCA) mechanism. Various protocols have been applied for detection of novel viruses, for differentiation between circular and linear forms of viral genomes and for generation of infectious genomic clones directly from specimens. Here, we summarize the broad application of the RCA technique to DNA viruses infecting humans, animals and plants.
ISSN: 0966-842X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical and Epidemiological Virology (Rega Institute)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science