Title: Kv Channel Gating Requires a Compatible S4-S5 Linker and Bottom Part of S6, Constrained by Non-interacting Residues
Authors: Labro, Alain J ×
Raes, Adam
Grottesi, Alessandro
Van Hoorick, Diane
Sansom, Mark S. P
Snyders, Dirk J #
Issue Date: Dec-2008
Publisher: Rockefeller univ press
Series Title: Journal of general physiology vol:132 issue:6 pages:667-680
Abstract: Voltage-dependent K+ channels transfer the voltage sensor movement into gate opening or closure through an electromechanical coupling. To test functionally whether an interaction between the S4-S5 linker (L45) and the cytoplasmic end of S6 (S6 T) constitutes this coupling, the L45 in hKv1.5 was replaced by corresponding hKv2.1 sequence. This exchange was not tolerated but could be rescued by also swapping S6 T. Exchanging both L45 and S6 T transferred hKv2.1 kinetics to an hKv1.5 background while preserving the voltage dependence. A one-by-one residue substitution scan of L45 and S6 T in hKv1.5 further shows that S6 T needs to be alpha-helical and forms a "crevice" in which residues I422 and T426 of L45 reside. These residues transfer the mechanical energy onto the S6 T crevice, whereas other residues in S6 T and L45 that are not involved in the interaction maintain the correct structure of the coupling.
ISSN: 0022-1295
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Biological Psychology
× corresponding author
# (joint) last author

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