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Title: Characterization of mutations located in exon 18 of the CFTR gene
Authors: Vankeerberghen, A
Wei, L
Teng, Hui
Jaspers, Martine
Cassiman, Jean-Jacques
Nilius, Bernd
Cuppens, Harry #
Issue Date: Oct-1998
Publisher: Elsevier science bv
Series Title: FEBS Letters vol:437 issue:1-2 pages:1-4
Abstract: In order to get a better insight into the function of amino acid residues located in the second transmembrane domain of the cystic fibrosis transmembrane conductance regulator (CFTR) protein, all exon 18 mutations found in cystic fibrosis (CF) patients were characterized at the protein and at the electrophysiological level. Of the different mutations present in transmembrane helix 12 (M1137V, M1137R, I11139V and deltaM1140), and the intracytoplasmic loop connecting TM12 and NBD2 (D1152H and D1154G), only M1137R interfered with the proper maturation of the protein. Permeability studies performed after injection of the different wild-type and mutant cRNAs in Xenopus laevis oocytes indicated that the mutations did not alter the permeability sequence of the CFTR channels. The whole cell cAMP activated chloride currents, however, were significantly reduced for M1137V, I1139V, D1152H and D1154G and close to zero for deltaM1140, indicating that these mutations interfere with the proper gating of the chloride channels.
URI: 
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Laboratory of Ion Channel Research
Hepatology
Human Mutations and Polymorphisms Section (-)
Department of Cellular and Molecular Medicine - miscellaneous
Forensic Biomedical Sciences
# (joint) last author

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