Title: Assessment of cellular actin dynamics by measurement of fluorescence anisotropy
Authors: Spitz, Jean-Alexis * ×
Polard, Valérie *
Maksimenko, Andréi
Subra, Frédéric
Baratti-Elbaz, Catherine
Méallet-Renault, Rachel
Pansu, Robert B
Tauc, Patrick
Auclair, Christian #
Issue Date: Aug-2007
Publisher: Elsevier
Series Title: Analytical Biochemistry vol:367 issue:1 pages:95-103
Abstract: To study cellular actin dynamics, a cell-free assay based on fluorescence anisotropy was developed. Using G-actin-Alexa as a probe, we found that anisotropy enhancement reflects F-actin elongation. Anisotropy enhancement varies with the concentration of magnesium and calcium cations and with ethylenediaminetetraacetate or well-known effectors of the polymerization. This assay gives the overall status of actin dynamics in cell extracts which are the closest conditions to in vivo, implying most of the regulating proteins that are missing in purified actin measurements. It can be used in a large-scale screening for chemical compounds which modulate actin polymerization.
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
* (joint) first author
× corresponding author
# (joint) last author

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