Title: Time-dependent protein expression modifications throughout area 17 of the retinal lesion cat
Authors: Hu, Tjing-Tjing
Van den Bergh, Gert
Eysel, Ulf T
Heylen, Kevin
Arckens, Lut #
Issue Date: 11-May-2009
Host Document: Acta Neurologica Belgica. Program and Abstract Book. pages:40
Conference: Bi-Annual Meeting of the Belgian Society for Neuroscience edition:8 location:Liège (Belgium) date:11 May 2009
Article number: 48
Abstract: Central retinal lesions lead to loss of visual input in the central part of area 17 of the adult cat. Eventually this results in topographic map reorganization within the sensory-deprived cortical lesion projection zone (LPZ) as measured by electrophysiology. This recovery coincides with changes in gene/protein expression. We therefore investigated these alterations as a function of post-lesion survival time in three regions of primary visual area 17: the center of the LPZ, the border of the LPZ and far peripheral area 17.
We analysed the differential protein expression patterns between control and experimental animals with 2-dimensional differential gel electrophoresis. We have traced 74 differential spots for the three regions in area 17. Central retinal lesions clearly induced region-specific and time-dependent protein expression changes. Even the protein expression in far peripheral area 17 spared from a direct impact of the central retinal lesions differed from the corresponding region in normal subjects. We succeeded in identifying 93 % of the differential spots by means of mass spectrometry and software processing helped functional grouping within the list of proteins. This analysis allowed us to select two plasticity-related proteins for further validation experiments.
ISSN: 0300-9009
Publication status: accepted
KU Leuven publication type: AMa
Appears in Collections:Research Group Neuroplasticity and Neuroproteomics (-)
Animal Physiology and Neurobiology Section - miscellaneous
# (joint) last author

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