Acta Neurologica Belgica. Program and Abstract Book. pages:40
Bi-Annual Meeting of the Belgian Society for Neuroscience edition:8 location:Liège (Belgium) date:11 May 2009
Central retinal lesions lead to loss of visual input in the central part of area 17 of the adult cat. Eventually this results in topographic map reorganization within the sensory-deprived cortical lesion projection zone (LPZ) as measured by electrophysiology. This recovery coincides with changes in gene/protein expression. We therefore investigated these alterations as a function of post-lesion survival time in three regions of primary visual area 17: the center of the LPZ, the border of the LPZ and far peripheral area 17.
We analysed the differential protein expression patterns between control and experimental animals with 2-dimensional differential gel electrophoresis. We have traced 74 differential spots for the three regions in area 17. Central retinal lesions clearly induced region-specific and time-dependent protein expression changes. Even the protein expression in far peripheral area 17 spared from a direct impact of the central retinal lesions differed from the corresponding region in normal subjects. We succeeded in identifying 93 % of the differential spots by means of mass spectrometry and software processing helped functional grouping within the list of proteins. This analysis allowed us to select two plasticity-related proteins for further validation experiments.