Title: Realignment process of actin stress fibers in single living cells studied by focused femtosecond laser irradiation
Authors: Yasukuni, Ryohei ×
Spitz, Jean-Alexis
Meallet-Renault, Rachel
Negishi, Takayuki
Tada, Takuji
Hosokawa, Yoichiroh
Asahi, Tsuyoshi
Shukunami, Chisa
Hiraki, Yuji
Masuhara, Hiroshi #
Issue Date: May-2007
Publisher: New York
Series Title: Applied Surface Science vol:253 issue:15 pages:6416-6419
Conference: 5th International Conference on Photo-Excited Processes and Applications location:Charlottesville, Virginia, USA date:3 Sept 2006
Abstract: Three-dimensional dissection of a single actin stress fiber in a living cell was performed based on multi-photon absorption of a focused femtosecond laser pulse. The realignment process of an actin stress fiber was investigated after its direct cutting by a single-shot femtosecond laser pulse irradiation by high-speed transmission and fluorescence imaging methods. It was confirmed that mechanical force led by the femtosecond laser cutting propagates to entire cell through the cytockelton in a 100 mu s time scale. The cut actin stress fiber was realigned in the time scale of a few tens of minutes. The dynamic analysis of the realignment induced by single-shot femtosecond laser gives new information on cell activity. (C) 2007 Elsevier B.V. All rights reserved.
ISSN: 0169-4332
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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