Title: Human cardiac mesoangioblasts isolated from hypertrophic cardiomyopathies are greatly reduced in proliferation and differentiation potency
Authors: Gálvez, Beatriz G ×
Covarello, Diego
Tolorenzi, Rosanna
Brunelli, Silvia
Dellavalle, Arianna
Crippa, Stefania
Mohammed, Salman Afroze Azmi
Scialla, Ludovica
Cuccovillo, Ivan
Molla, Fabiola
Staszewsky, Lidia
Maisano, Francesco
Sampaolesi, Maurilio
Latini, Roberto
Cossu, Giulio #
Issue Date: Sep-2009
Publisher: British Medical Association
Series Title: Cardiovascular Research vol:83 issue:4 pages:707-716
Abstract: AIMS: Our objective was to test whether progenitor cell proliferation and differentiation potential may vary depending upon the disease of the donor. METHODS AND RESULTS: Human cardiac mesoangioblasts were isolated from cardiac muscle biopsies of patients undergoing open heart surgery for correction of mitral regurgitation following an acute myocardial infarction (MR-MI) or correction of mitral and aortic regurgitation with ensuing left ventricular hypertrophy (MAR-LVH). The cells express surface markers and cardiac genes similar to mouse cardiac mesoangioblasts; they have limited self-renewing and clonogenic activity and are committed mainly to cardiogenesis. Although cardiac differentiation can be induced by 5-azacytidine or by co-culture with rat neonatal cardiomyocytes, human cells do not contract spontaneously like their mouse counterparts. When locally injected in the infarcted myocardium of immunodeficient mice, cardiac mesoangioblasts generate a chimeric heart that contains human myocytes and some capillaries; likewise, they colonize chick embryo hearts when transplanted in ovo. At variance with cells from patients with MR-MI, when isolation was performed on biopsies from MAR-LVH, cells could be isolated in much lower numbers, proliferated less extensively and failed to differentiate. CONCLUSION: Cardiac mesoangioblasts are present in the human heart but this endogenous progenitor population is progressively exhausted, possibly by continuous and inefficient regeneration attempts.
ISSN: 0008-6363
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Interdepartemental Stem Cell Institute (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Galvez - Cardiovascular Research - 2009.pdf Published 541KbAdobe PDFView/Open


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science